1) What was the main question asked in Kaiser et al.? 2) What is the hypothesis tested in the experiment for Fig. 2? What was their prediction if this hypothesis is supported? And was it supported? 3

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1) What was the main question asked in Kaiser et al.?

2) What is the hypothesis tested in the experiment for Fig. 2? What was their prediction if this hypothesis is supported? And was it supported?

3) Why do leaves often lose their green color in the fall? And why are yellow leaves considered a poor nutrient source?

4) Why did the authors use universal and group-specific primers? What’s the difference between these two?

5) Describe the compensatory larval feeding by the G2 larvae of 1% antibiotic treated G0 (Fig. 3b and c).

6) What are three hypotheses for how Wolbachia symbionts might induce green-islands?

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