You perform a series of experiments on the synthesis of the pituitary hormone prolactin, which is a…

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You perform a series of experiments on the synthesis of the pituitary hormone prolactin, which is a… Show more You perform a series of experiments on the synthesis of the pituitary hormone prolactin, which is a single polypeptide chain 199 amino acids long. in the first experiment, the mRNA coding for prolactin is translated in a cell free protein synthesizing system containing ribosomes, amino acids, tRNAs, aminoacyl-tRNA synthetases, ATP, GTP and the appropraite initiation, elongation and termination factors. Under these conditions, a polypeptide chain 227 amino acids long is synthesized. In a second experiment, the mRNA is translated in the same cell-free system to which you have added SRP. In this case, translation stops after a peptide about 70 amino acids long has been produced. In a third experiment, you ise the same cell free translation system to which you have added both SRP and endoplasmic reticulum vesicles, and you fing that the prolactin mRNA no prduces a polypeptide that is 199 amino acids long. Explain these results. Where would you find the polypeptide produced in experiment 3? • Show less

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