You have modified the green fluorescent protein (GFP) to carry a nuclear localization signal at its…

Ace your studies with our custom writing services! We've got your back for top grades and timely submissions, so you can say goodbye to the stress. Trust us to get you there!


Order a Similar Paper Order a Different Paper

You have modified the green fluorescent protein (GFP) to carry a nuclear localization signal at its… Show more You have modified the green fluorescent protein (GFP) to carry a nuclear localization signal at its N-terminus and a nuclear export signal at its C-terminus. When this protein is expressed in cells, you find that about 60% of it is located in the nucleus and the rest in the cytoplasm and that its rates of import and export determine the observed distribution. Nevertheless, you are concerned that the modified GFP might not fold properly even though it retains its fluorescent properties. Specifically, you worry that the 40% in the cytoplasm is not being transported at all because neither signal is exposed, and the 60% in the nucleus has only the nuclear import signal available. To test this, you may treat cells with leptomycin B, a drug that inhibits nuclear export. How might addition of leptomycin B to such cells resolve this issue? • Show less

Writerbay.net

Looking for top-notch essay writing services? We've got you covered! Connect with our writing experts today. Placing your order is easy, taking less than 5 minutes. Click below to get started.


Order a Similar Paper Order a Different Paper