Hello. Attached I have 3 genetic labs that I need assistance with. In some of the questions, it says you’ll need to watch the video to answer the questions. This for the most part is not true. I usual

Hello. Attached I have 3 genetic labs that I need assistance with. In some of the questions, it says you’ll need to watch the video to answer the questions. This for the most part is not true. I usual
Watch th e vi deo: A nsw er t h e q uestio ns b elo w : 1. W hat ki nd o f se quenci ng te ch nolo gy has re ce ntly m ade it p ossi ble fo r sci entist s to fill in th e g aps of m issi ng se quence of th e ce ntr o m ere o n th e X -ch ro m oso me? 2. W hy does th e ce ntr o m ere o f a ch ro m oso me te nd to b e d ifficu lt to se quence ? 3. W hy did th e te am in th e vi deo ch oose th e X -ch ro m oso me fo r th eir r e se arch ? 4. W hy is th is re se arch im porta nt? S ie rra N etz -D ec 02, 2 021, 1 0:3 0 A M M ST First Complete Human X Chr omosome Sequence: In Conv ersation wiFirst Complete Human X Chromosome Sequence: In Conv ersation wi …… 5. W hy is it im porta nt to ca ta lo g g enom ic va ria tio ns in m ultip le in divi duals? 6 . H ow m ig ht th is re se arch in flu ence th e m edica l fie ld in th e fu tu re ?
Hello. Attached I have 3 genetic labs that I need assistance with. In some of the questions, it says you’ll need to watch the video to answer the questions. This for the most part is not true. I usual
by Ghizl ane B endriss, A li C haari, a nd K uei- C hiu C hen P re m edica l D ivi sion, W eill C orn ell M edici ne–Q ata r, D oha, Q ata r N ATIO NAL C EN TER F O R C ASE S TU DY T EA CHIN G IN S C IE N CE P art I – F lu ore sce nt d dN TP s Sara , a co lle ge so phom ore m ajo rin g in b io lo gica l sci ence , w as both e xci te d a nd w orrie d. S he h ad b een a w ard ed a r e se arch in te rn sh ip in a la b w hose p ro je ct w as to fin d th e sp eci es co mposi tio n o f a st udy site n ear ca mpus. H er m ain a ct ivi ty would b e th e D NA se quenci ng o f sp eci fic genes use d fo r sp eci es id entifica tio n. B ut it h ad b een a w hole ye ar si nce sh e fir st le arn ed th e to pic of D NA se quenci ng fr o m h er in tr o duct ory bio lo gy class; h ow m uch d id sh e r e ally r e m em ber? S o, o n a T hursd ay afte rn oon, sh e o pened h er o ld n ote s and r e vi ew ed th e p ara gra phs sh e h ad w ritte n a bout S anger se quenci ng: T he m ost w id ely use d D NA se quenci ng te ch niq ue w as in ve nte d b y Fre derick Sanger b ase d in C am brid ge, E ngla nd, and h is co lle agues, P aul B erg a nd W alte r G ilb ert. T hey use d th e syn th esi ze d d id eoxyn ucl eotid es to te rm in ate th e ch ain o f D NA e lo ngatio n. T his new te ch niq ue is base d o n th e se le ct ive in co rp ora tio n o f ch ain -te rm in atin g d id eoxyn ucl eotid es, also kn ow n a s 2′, 3 ‘ d id eoxyn ucl eotid es, a nd a bbre vi ate d a s ddN TP s (d dG TP, d dA TP, d dT TP a nd d dC TP ). T he e xt ensi on r e act io n is divi ded b etw een fo ur d iffe re nt te rm in atio n r e act io ns, each co nta in in g a r a dio act ive n ucl eotid e th at ca n b e in co rp ora te d b y th e D NA p olym era se d urin g th e e xt ensi on st ep. B eca use th e d id eoxyn ucl eotid es la ck a h yd ro xyl g ro up o n th e 3 ́ ca rb on o f th e su gar r in g, th e a dditio n o f a n ucl eotid e ca nnot co ntin ue, a nd th is m arks th e e nd o f th e e lo ngatio n. T he r e su lt- in g fr a gm ents ca n th en b e r e so lve d o n a h ig h- re so lu tio n fo ur-la ne-w id e a nd r a dio act ive p olya crylam id e g el th at r e ve als th e co mple m enta ry se quence ( F ig ure 1 ). S anger, B erg a nd G ilb ert w on th e N obel P rize o f C hem ist ry in 1 980 fo r th e “ d id eoxy meth od” a lso kn ow n a s “S anger m eth od” o r th e “ fir st g enera tio n se quenci ng” ( se e S anger e t a l., 1 977). L ate r, P ro fe sso r H ood’s te am a t C alte ch d eve lo ped th e te ch niq ue, in w hich th e r a dio act ive n ucl eotid e w as re pla ce d b y flu ore sce nt la bels and in cl uded th e u se o f a la se r to d ete ct th e flu ore sce nt co lo rs. T he e le ct ro phore tic pro ce ss is co nnect ed to a co mpute r fo r th e d isp la y of th e r e su ltin g se quence . T hey publish ed th eir w ork in N atu re ( se e S m ith e t a l., 1 986). T his work was fu rth er im pro ve d b y th e flu ore sce nt la belin g o f d dN TP s by a te am o f r e se arch ers at th e D uP ont R ese arch S ta tio n a nd th e te ch niq ue w as descr ib ed in a n a rticl e le d b y Dr. Ja mes Pro ber in th e jo urn al S ci ence ( se e P ro ber e t a l., 1 987). S ie rra N etz -D ec 03, 2 021, 1 1 :0 0 A M M ST Figur e 1 . A utor adi ogr aph f rom four sepa ra te di deoxy s eque ncing r eactions . On h er fir st d ay of r e se arch w ork, S ara m et w ith th e p ost doct ora l r e se arch er, D r. D ana, w ho w ould g o o ve r th e d eta ils of her ta sks with h er. D r. D ana sm ile d a t h er n ew in te rn . “ W elco me S ara ! A re yo u e xci te d fo r yo ur fir st d ay in la b?” “H i, D r. D ana,” S ara r e plie d. “ I’m r e ally exci te d to g et st arte d, a lth ough I’m a little w orrie d b eca use I’ve n eve r d one se quenci ng e xp erim ents befo re . A lso , I’ve n eve r u se d th ose se quenci ng m ach in es, so I’m a b it a nxi ous. ” Dr. D ana n odded w ith u nderst andin g. “ W ell, I h ave g ood a nd b ad n ew s. I’ll st art w ith th e b ad; o ur se quenci ng m ach in e is u nder r o utin e m ain te nance to day, b ut it sh ould b e w orki ng b y to m orro w m orn in g. T he g ood n ew s is th at yo u w ill n ot w ast e yo ur tim e to day, b eca use yo u’ll b e p re parin g th e r e act io n tu bes to b e u se d to m orro w .” S ara r e ce ive d th e p ro to co l a nd th e m ate ria l fr o m D r. D ana a nd st arte d h er b ench w ork. S he w ro te in h er la b n ote book th e in gre die nts fo r th e e xp erim ent. T he D NA sh e w as tr yi ng to se quence w as 3 ́A TC GGTA C AAG GTC G5 ́ w ith th e fir st five b ase s use d fo r p rim er b in din g. Exp erim ent 1 : • 0.5 pmole DNA template • 0.5 pmole primer • 1 mL of 10× Taq polymerase • 80 mM dNTPs (equal portion of dATP, dTTP, dGTP, dCTP) • 8 mM ddNTPs each – – – – Total: 10 ml volume A s sh e g ently added a nd m ixe d a ll o f th e co mponents to geth er S ara w ondere d w hat w as act ually happenin g w ith in th e tu be. S he w ent o nlin e a nd w atch ed th e fo llo w in g vi deo to r e m in d h erse lf o f th e p rin ci ple s of D NA se quenci ng: S ara ca lle d D r. D ana to le t h er kn ow th at sh e h ad fin ish ed p re parin g th e tu be fo r cycl e se quenci ng. D r. D ana w as clearly p le ase d. “ T hat’s gre at, S ara , yo u’r e fa st ! W e’ll p ut th at o n th e th erm al cycl er a nd in th e m eantim e r u n a tr a ditio nal se quenci ng, ju st to g et a n id ea o f th is sh ort se quence . W e’ll th en co mpare th is to th e ch ro m ato gra m to m orro w o nce th e m ach in e is up a nd r u nnin g to co nfir m th e se quence . W hat d o yo u th in k? ” “S ure ,” r e plie d S ara . “ B ut fir st , w hat d o yo u m ean e xa ctly by ‘tr a ditio nal se quenci ng’? ” “O h! I’m su re yo u’ve st udie d th at b efo re . S equenci ng g els have n’t a lw ays been co lo rfu l. In th e o rig in al S anger se quenci ng m eth od, n o o ne co uld te ll o ne fr a gm ent a part fr o m a noth er. S o, in o rd er to b e a ble to fig ure o ut th e se quence , p eople h ad to m ake su re th e fo ur r e act io ns were se t u p in fo ur se para te tu bes and th ey had to kn ow w hich tu be h ad w hich d id eoxyn ucl eotid e.” DN A Sequencing: The Chain T ermination Method (Sanger Method)DNA Sequencing: The Chain T ermination Method (Sanger Method) Sara n odded. “ S o, d o yo u m ean I’ll h ave to p re pare fo ur d iffe re nt tu bes and lo ad th em in fo ur d iffe re nt w ells la te r? ” “E xa ctly ,” r e plie d D r. D ana. “ A nd p le ase m ake su re yo u w rite e ve ryt hin g yo u d o in yo ur n ote book. ” Sara h eaded b ack to th e b ench . S he u se d th e sa me te m pla te D NA 3 ́A TC GGTA C AAG GTC G5 ́ w ith th e fir st five b ase s use d fo r p rim er b in din g. S he st arte d h er w ork but w as a little d ist ra ct ed b y th e m usi c sh e w as list enin g to . W hen sh e notice d th at sh e h ad m ade a m ist ake in th e p ro ce dure , sh e r e co rd ed th e e rro r in h er n ote s: E xp erim ent 2 : • 0.5 pmole DNA template • 0.5 pmole radioactive primer • 10 mL of 10X Taq polymerase • 80 mM dNTPs (equal portion of dATP, dTTP, dGTP, dCTP) • 8 mM ddNTPs each, separately – ddATP** (Oops! I forgot to add ddATP in the A tube!) – ddGTP – ddTTP – ddCTP Total in 10 mL volume A nsw er t h e q uestio ns b elo w : 1. H ow m any re act io n tu bes does Sara n eed to p re pare in th is exp erim ent? 2 . W hat sh ould e ach r e act io n tu be co nta in ? 3. F ollo w th e e xa mple in th e le ft p anel o f F ig ure 4 b elo w to co mple te th e g el p ict ure in th e r ig ht p anel. a nd u plo ad w hat it w ould lo ok like ) T he D NA b ands sh ould co rre sp ond to th e fr a gm ent si ze s sh ow n o n th e le ft si de o f th e p anel a nd r e fle ct th e r e su lts fr o m th e a bse nce o f d dA TP in o ne o f th e r e act io ns. Fig ure 4 . G el im age fr o m E xp erim ent 2 . U se th e le ft p anel a s an e xa mple fo r fillin g in th e r ig ht p anel. a “ ? ” m ark to in dica te it.
Hello. Attached I have 3 genetic labs that I need assistance with. In some of the questions, it says you’ll need to watch the video to answer the questions. This for the most part is not true. I usual
Exp erim ent 2 , T a ke T w o Sara r e alize d th at sh e st ill h ad so me tim e b efo re D r. D ana ca me b ack. S he w ante d to d o it r ig ht th is tim e, m aki ng su re to a dd a ll typ es of d dN TP s. W hen th e r e act io n r u n co mple te d th is tim e, sh e p ro duce d th e g el p ict ure a s in F ig ure 5 . F ig ure 5 . G el im age fr o m E xp erim ent 2 .2 . S ara sh ow ed h er tw o g els to D r. D ana, a little b it e m barra sse d th at sh e h adn’t b een a ble to p ro duce a co rre ct g el w ith th e tr a ditio nal m eth od. D r. D ana w ave d it a si de b y sa ying, “ N o w orrie s! T his typ e o f m ist ake h appens all th e tim e. T he m ost im porta nt th in g is to ke ep ta ki ng g ood n ote s in yo ur la b n ote book so w e ca n tr o uble sh oot a nd u nderst and w hat m ust h ave h appened. I’m h appy to se e yo u d id th at. P le ase g et so me r e st a nd I’ll se e yo u to m orro w m orn in g a s th e m ach in e is now r e ady.” T he n ext d ay, S ara b ro ught h er r e act io n tu be to r u n w ith th e se quenci ng m ach in e. T he D NA fr a gm ents in S ara ’s tu be w ere sca nned b y th e m ach in e a nd r e ad b y a co mpute r. F ig ure 6 is th e ch ro m ato gra m o bta in ed. Sie rra N etz -D ec 03, 2 021, 1 1 :1 0 A M M ST Fig ure 6 . C hro m ato gra m p ro duce d fr o m S ara ’s exp erim ent. A nsw er t h e q uestio ns b elo w : 5. K now in g th at S ara h as added a ll th e in gre die nts, w hat co uld b e a r e aso n fo r m issi ng so me o f th e b ands? (H in t: co nsi der th e q uantity of th e in gre die nts. ) 6. 7 . Is th e d ir e ct io n o f th e ch ro m ato gra m fr o m le ft to r ig ht 5 ́ to 3 ́ o r 3 ́ to 5 ́ o f th e se quence ? 8. W hat d oes th e h eig ht o f a p eak in dica te ? ( H in t: co nsi der th e b rig htn ess or th ickn ess of a D NA b and in a n ele ct ro phore tic gel.) 9 . D o yo u th in k Sara w as able to o bta in th e se quence o f th e p rim er fr o m th e d id eoxy sequenci ng m eth od? E xp la in . 10. T he flu ore sce nt la bel in ve ntio n w as deve lo ped b y Pro fe sso r H ood’s gro up a t C alifo rn ia In st itu te o f T e ch nolo gy. B ut th ey still h ad to ca rry out se quenci ng in fo ur se para te r e act io n tu bes, u ntil a noth er te am , in th e D uP ont R ese arch L ab, fu rth er d eve lo ped th e te ch niq ue. W hat sp eci fics about th e la belin g a llo w se quenci ng to b e co nduct ed in a si ngle r e act io n tu be? C oncl usi on Dr. D ana w as ve ry ple ase d w ith th e r e su lts and co ngra tu la te d h er n ew in te rn . “ W ell d one S ara ! W e’ve g ot a n ice ch ro m ato gra m w ith m in im um n oise .” S ara sm ile d, r e lie ve d th at sh e h ad b een a ble to r e co ver fr o m th e m ist ake sh e h ad m ade ye sterd ay. S he a lso n ow h ad a b ette r u nderst andin g o f h ow flu ore sce nce a llo w ed a uto m atio n o f se quenci ng, a nd h ow h er w ork was gre atly fa ci lita te d by th e a bility to ca rry out se quenci ng in a si ngle tu be. T he in itia l w orrie s sh e h ad fe lt a bout h er n ew in te rn sh ip w ere g one, a nd n ow o nly exci te m ent r e m ain ed.
Hello. Attached I have 3 genetic labs that I need assistance with. In some of the questions, it says you’ll need to watch the video to answer the questions. This for the most part is not true. I usual
Watc h th is v id eo a nd a nsw er th e q uestio ns. h ttp s://w ww.n ew s-m edic al. n et/lif e -s cie nces/Q uoru m -S ensin g-a nd-V ib rio -C hole ra e.a spx A nsw er th e fo llo w in g q uestio ns: 1. W hat is q uoru m s ensin g? 2. W hat a re tw o p ossib le a pplic atio ns fo r u sin g q uoru m s ensin g in m edic in e? 2. W hat d o th e e nzym es p ro duced b y th e lu x o pero n d o? S ie rra N etz -M ar 2 5, 2 021, 1 0:4 5 A M M DT
Hello. Attached I have 3 genetic labs that I need assistance with. In some of the questions, it says you’ll need to watch the video to answer the questions. This for the most part is not true. I usual
Pra ctic e A nnota tio n. Y ou m ay n eed y our R eadin g P rim ary L it e ra tu re b ookle t a s a re fe re nce. T here is a lis t o f te rm s in clu ded th at m ay b e u sefu l in h elp in g y ou u nders ta nd th is s hort p aper. P le ase w rite a s am ple a n nota tio n fo r th e lu x o pero n p ap er b elo w th at in clu des th e fo llo w in g: a. F ull lis t o f a uth ors , fu ll tit le a nd jo urn al c it a tio n (y ear, jo urn al n am e, v olu m e, p age, n um bers ; y ou m ust fo llo w th e A PA c it a tio n s ty le (P urd ue O wl has a g re at s it e fo r e xpla in in g A PA s ty le : h ttp s://o w l. e nglis h.p urd ue.e du/o w l/ re sourc e/5 60/0 1/ if y ou n eed a re fre sher) b . th e p aper is a ddre ssin g (i. e . th e b ro ader re le vance; n ote th at s om etim es th e a uth ors th em selv es m ay n ot s ay m uch a bout th is .) T he g oal h ere is t o d ra w in y our re ader s o w e w ant to re ad th e re st o f th e a nnota tio n. c. S ie rra N etz -M ar 2 5, 2 021, 1 0:4 5 A M M DT d. e.
Hello. Attached I have 3 genetic labs that I need assistance with. In some of the questions, it says you’ll need to watch the video to answer the questions. This for the most part is not true. I usual
Watc h a t le ast th e fir s t 4 m in ute s o f th is v id eo to le arn a bout th e b asic p rin cip le s o f v ecto r c lo nin g fro m J oV E (s ig n in w it h C NM cre dentia ls ): h ttp s://w ww.jo ve.c om /v /5 074/m ole cula r-c lo nin g a nd th e fir s t 3 m in ute s o f th is v id eo to le arn th e b asic s a bout re stric tio n e nzym es: http s://w ww.jo ve.c om /v /5 070/re stric tio n-e nzym e-d ig ests B oth v id eos g o d eep in to d eta ils a bout th e p ro cesses a nd y ou d on’t n eed th at d epth o f in fo rm atio n, ju st th e b asic s. Y ou a re g oin g to s ee if it m ig ht b e p ossib le to u se a s pecif ic b acte ria l p la sm id , c alle d p U C19, to c lo ne a p ie ce o f th e h um an R FP p ro te in u sin g o ne or m ore re stric tio n e nzym es. Y ou w ill n eed : 1)th e re fe re nce s equence fo r th e fir s t 1 800 b ases (fro m N CBI B la st D ata base) in th e R FP p ro te in , (a tta chm ent) 2 ) th e lis t o f re stric tio n e nzym es (fo und a t th e b otto m o f th is p age) 3) th e s chem atic fo r b acte ria l c lo nin g p la sm id , p U C19 (a tta chm ent) L ook a t th e re stric tio n e nzym es o n th e lis t th at y ou h ave b een g iv en. T hese w ill a ll c ut s om ew here w it h in th e m ult ip le c lo nin g s it e (M CS) o n pU C19. O ut o f th e lis t, fin d fo ur re stric tio n e nzym es th at w ill c ut s om ew here w it h in th e 1 800 b ase-p air R FP re fe re nce s equence y ou a re g iv en. 1.) T he re fe re nce s equence is lis te d 5 ’- 3 ’. Y ou c an s earc h fo r re stric tio n s it e s o n th is s equence b y u sin g th e “F in d” fu nctio n in y our w ord p ro cessor. U se fo ur d if fe re nt c olo r h ig hlig hts to m ark o n y our R FP re fe re nce g ene s equence a ll th e s it e s w here e ach o f y our re stric tio n enzym es w ill c ut. F or e xam ple , to fin d th e p la ces w here E coR 1 w ould c ut o n th e R FP, y ou w ould c opy th e E coR 1 re stric tio n re cognit io n s it e fro m th e lis t ( G AATTC ) a nd th en p aste it in to th e “fin d” fu nctio n o n y our w ord p ro cessor in th e re fe re nce s equence. E very s it e w here th is s equence is fo und w ill s how u p a nd th en y ou c an h ig hlig ht th em . T ry th is w it h e very re stric tio n s it e lis te d. 2.) N am e o f R E 1. 2. 3. 4. 3.) L ook fo r s om eth in g th at c uts c lo se to th e b egin nin g a nd s om eth in g th at c uts c lo se to th e e nd. 4.) L is t o f r e str ic tio n e n zym e r e co gnitio n s eq uen ces fr o m Wik ip ed ia (a ll a re lis te d in 5 ’  3 ’ d ir e ctio n) a ll o f th ese a re fo und w ith in th e pU C19 M CS: Acc65I- G GTACC A poI- A ATT B spM I- A CCTGC B am HI- G GATCC Sie rra N etz -M ar 2 5, 2 021, 1 0:4 5 A M M DT EcoR I- G AATTC H in dIII- A AGCTT P stI- C TGCAG S alI – G TCGAC S acI- G AGCTC S bfI- C CTGCAGG S m aI- C CCGGG S phI- G CATGC
Hello. Attached I have 3 genetic labs that I need assistance with. In some of the questions, it says you’ll need to watch the video to answer the questions. This for the most part is not true. I usual
Muta tio ns: P le ase a n sw er th e b elo w q uestio ns. 1. G iv en a n m RNA w it h th e fo llo w in g s equence, p le ase tra nsla te th e c odons to a c hain o f a m in o a cid s. U se th e c odon c hart lin ked h ere : http s://s im ple m ole cula rg enetic s.w eebly .c om /u plo ads/1 /8 /3 /4 /1 8345767/5 269678_orig .p ng 5’A UG /C CU/G CU/U AC/C G G/G AG /U AA3’ “m et-_ ________-_ ________-_ ________-_ ________-_ ________”S TO P” 2. W e le arn ed a bout fo ur ty pes o f p oin t m uta tio ns th at o ccur o n D NA. A ssum in g th e o rig in al p oly peptid e c hain b elo w , m atc h e ach ty pe o f p oin t m uta tio n w it h th e p oly peptid e c hain th at re sult s . O rig in al p oly p ep tid e: P ro -T hr-S er-L eu -L eu -H is -A sn A . M is sense B . Sile nt C . Nonsense D . Fra m eshif t _ _____ P ro -T hr-S er-S TO P ______ P ro -T hr-S er-L eu-Ile -H is -A sn ______ P ro -T hr-S er-L eu-L eu-H is -A sn _______ P ro -T hr-H is -C ys-T yr-T hr S ie rra N etz -M ar 1 2, 2 021, 1 0:5 2 A M M ST
Hello. Attached I have 3 genetic labs that I need assistance with. In some of the questions, it says you’ll need to watch the video to answer the questions. This for the most part is not true. I usual
Watc h th ese th re e v id eos: K han A cadem y: O pero ns a nd G ene R egula tio n in B acte ria K han A cadem y: T he la c o pero n K han A cadem y: T he trp o pero n A fte r y o u w atc h th e v id eo s, p le ase a n sw er th e q uestio ns b elo w : 1. D efin e th e fo llo w in g in th e c onte xt o f o pero ns: a. p osit iv e c ontro l b. g iv e a n e xam ple o f a n o pero n th at is u nder p osit iv e c ontro l c. n egativ e c ontro l d. g iv e a n e xam ple o f a n o pero n th at is u nder n egativ e c ontro l e. re pre ssor f. g iv e a n e xam ple o f a re pre ssor m ole cule g . a ctiv ato r h. g iv e a n e xam ple o f a n a ctiv ato r 2. D ra w a p ic tu re o f w hat th e la c o pero n w ould lo ok lik e in a s it u atio n w here g lu cose is n ot p re sent b ut la cto se is . Y ou m ust in clu de th e fo llo w in g: la cZ g ene, la cY g ene, la cA g ene, o pera to r, p ro m ote r, R NA p oly m era se, C AP s it e , C AP p ro te in , re pre ssor (e it h er b ound o r u nbound) a nd allo la cto se. In dic ate w heth er tra nscrip tio n is h appenin g o r n ot. D o n ot c opy/p aste a p ic tu re fro m th e in te rn et, y ou m ust u se y our o w n w ork a nd uplo ad it o r d ra w it u sin g a n o nlin e p ro gra m . Sie rra N etz -M ar 1 2, 2 021, 1 0:5 2 A M M ST 3. D ra w a nd u plo ad a p ic tu re o f w hat th e trp o pero n w ould lo ok lik e in a s it u atio n w here trp is re adily a vaila ble to th e c ell. Y ou m ust in clu de th e 5 s tru ctu ra l g enes o f th e o pero n( trp A -E in c orre ct o rd er), o pera to r, re pre ssor, R NA p oly m era se, try pto phan a nd in dic ate w heth er tra nscrip tio n is h appenin g. D o y our o w n w ork .
Hello. Attached I have 3 genetic labs that I need assistance with. In some of the questions, it says you’ll need to watch the video to answer the questions. This for the most part is not true. I usual
Human RFP reference sequence (from NCBI) 1 gaattcacaaaccctgagctagacacagggtgctgattggtgtgtttacaaaccttgagc 61 tagatacagagtgccgattggtgtatttacaatcccttcgctagacataaaggttctcca 121 agtccccaccagagtagctaaatacagagtgtccattggtgcattcacaaaccctgagct 181 agacacagggtgccgattggtgtgtttacaaaccttgagctagatacagagtgccgattg 241 gtgtatttataatcccttagctagacgtaaacgttctccaagtccctaccagactcagga 301 gcccagctggcttcacccagtggattttccaccggtgccgcaggtggagctgcctgccag 361 tcccgtgctttgcgcccgcacttctcagccgttgggtggccgatgggattgggcgccgtg 421 gagcagggggcggcgctcgtcggggaggctcgggccgcgcaggagcccatggcggggagg 481 ggcgtctcaggcatggcgggctgtaggtcccgagccttaccccgcggggagacagctaag 541 gcccggcgagaagtcgagaacagcagctgctggcacaggtgctaagcctcttactgctcg 601 gggcttgcggattagggggccgctccgagtgcggagcccgccgagcccacgcccacccgg 661 aactcgcgctgggcccgcaagcgccgcgcgcagcctcggttcccgcctgcgcttctccct 721 ccacacatccctgcaagctgagggagccggctccggccttggccagcccagcaaggggct 781 cccacagtgcagcggcgggctgaagttctcctcaagcgcggccagagtgggcgccaaggc 841 cgaggaggcgcctagagcaagcgaaggctgtgagggctgccagcaagctgtcacctctca 901 gtatggcggctggctgttttagcaccatgtcgtttattgtcatgcatttgtaggattatg 961 aaatgcttcctgaattttgcttttacagtaacttgtattcattcatgcatttttcaacct 1021 gctcactccagttcaaggtctttggtggctgaagcctaattcaactcctcatagtgtcag 1081 gagggaacccaccgtggacaggtggccattccatcacagggcgggctcatacacacacac 1141 acacactcacacatatgctcgcgtgctctttcactcagactgatgacgctagactcagac 1201 tagatatgctaatgaacctaatgtgcacatttttgggatgtgggaggaaactcagacagt 1261 ggcttccaggagaaatagacttttttctcatcaacattataacaaaatgatgttgaatga 1321 aacaacgttattcaagggtctgctgtacgcagattttcctatttctttaggtcttcattt 1381 ttgaaggctcttgtgtcaataaaatttgtttgatttgtatgcttttcctttttttttttt 1441 tttttttttgttgttgagacagaatttcacttttgttgcccaggctagagtgtaatggcg 1501 cgatcttggctcaccacaacctccgcctcccgggttcaagcgattcttctgcttcagcct 1561 cccgagtagctgggattacaggcgtgtgccactatgcccagctaatttcgtatttttagt 1621 ggaaatgggggtttctccatgttggccaggctggtctcaaactcctgacctcaggtgatc 1681 caccagcttcagccccccaaagtgctgggattacaggcatgagccaccccacccggcctg 1741 cttttcccttgttaatctatcttttattatgaagtgtcagccatgaacctggcactgggt 1801 gggaaaagatgtttttctgccctagaccttcctataagtgcttttgggacaacactgcag